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Ameliorative Effects of Thymoquinone on Sperm Parameters and Testosterone Level of Nicotine-Treated Sprague Dawley Rats BABT
Rosli,Farah Dayana; Hashim,Noor Hashida; Osman,Khairul; Ibrahim,Siti Fatimah; Kabir,Nurul.
Abstract Thymoquinone (TQ), the main constituent of the volatile oil derived from Nigella sativa has shown pharmacological benefits against various diseases while nicotine is an active component in cigarette that is known to be detrimental. This study was conducted to assess the ameliorating effects of TQ on sperm count, membrane, mitochondria and testosterone of nicotine-treated rats. Rats were randomized into four groups: control, nicotine, TQ, and nicotine with TQ. Nicotine (5 mg/kg bwt/day) was subcutaneously injected for 30 days to induce damaging effects on sperm and testosterone level. Rats were force-fed with TQ (5 mg/kg bwt/day) for the following 30 days. Sperm count was reduced in the nicotine group (26.72 ± 1.64 106/mL) but showed a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thymoquinone; Nicotine; Rat sperm; Membrane; Mitochondria; Testosterone.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100304
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Development of single tubing-type canister for cryo-storage of bull semen and their effect on sperm motility and viability BABT
Ismail,Mohd Iswadi; Osman,Khairul; Ibrahim,Siti Fatimah; Jaafar,Farah Hanan Fatihah; Ghani,Nur Azianie Abd; Zainalabidin,Fazly Ann; Othman,Abas Mazni.
The objective of this study was to evaluate the potential of using single tubing-type canister on sperm quality. Semen was collected from the Bali cattle bull by electroejaculation technique and was cryopreserved in liquid nitrogen using slow freezing cryopreservation method. Two type of canister volume was used in this study; commercial canister (342.25π x 278 mm²) and single tubing-type canister (4π x 90 mm²). Makler counting chamber and computer assisted sperm analyzer (CASA) were used to evaluate the sperm motility and viability of post-thaw sperm. Results showed that the bull sperm motility and viability at the bottom of tubing-type canister was statistically higher and significant as compared to the commercial canister (p<0.05). Significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryopreservation; Sperm; Canister; Motility; Viability.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200006
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